Direct and sensitive miRNA profiling from low-input total RNA
نویسندگان
چکیده
منابع مشابه
Multithreaded Input-Sensitive Profiling
Input-sensitive profiling is a recent performance analysis technique that makes it possible to estimate the empirical cost function of individual routines of a program, helping developers understand how performance scales to larger inputs and pinpoint asymptotic bottlenecks in the code. A current limitation of input-sensitive profilers is that they specifically target sequential computations, i...
متن کاملHigh-throughput stem-loop RT-qPCR miRNA expression profiling using minute amounts of input RNA
MicroRNAs (miRNAs) are an emerging class of small non-coding RNAs implicated in a wide variety of cellular processes. Research in this field is accelerating, and the growing number of miRNAs emphasizes the need for high-throughput and sensitive detection methods. Here we present the successful evaluation of the Megaplex reverse transcription format of the stem-loop primer-based real-time quanti...
متن کامل114 A Highly Sensitive and Specific Universal Mirna Profiling Method
helminthic infections, and allergy. They are closely associated with recruiting and amplifying T helper 2 (Th2) lymphocyte response in contrast to Th1asscoiated CAMs. Wide donor-to-donor variability of human primary monocytes and their limited life span in vitro is a current impediment to investigating human AAM biology and their contribution to enhancing Th2mediated pathologic inflammation fou...
متن کاملLabel-free high-throughput microRNA expression profiling from total RNA
MicroRNAs (miRNAs) are key biological regulators and promising disease markers whose detection technologies hold great potentials in advancing fundamental research and medical diagnostics. Currently, miRNAs in biological samples have to be labeled before being applied to most high-throughput assays. Although effective, these labeling-based approaches are usually labor-intensive, time-consuming ...
متن کاملmiRNA profiling from blood — challenges and recommendations
The discovery of stable miRNA species circulating in blood has led to increased research focus on disease-related variations in serum and plasma miRNA expression and the possibility that such variations could serve as noninvasive biomarkers for disease. Working with serum and plasma miRNA presents various challenges in purification and characterization. In this paper, we outline QIAGEN® recomme...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: RNA
سال: 2006
ISSN: 1355-8382
DOI: 10.1261/rna.234507